Stage M2: The role of miRNA in myogenic cell communication H/F
Société : Institut de Radioprotection et de Sûreté Nucléaire Lieu : Hauts-de-Seine (Île-de-France)
Présentation de l'entreprise
L'IRSN, Etablissement Public à caractère Industriel et Commercial (EPIC) - dont les missions sont désormais définies par la Loi n° 2015-992 du 17 août 2015 relative à la transition énergétique pour la croissance verte (TECV) - est l'expert public national des risques nucléaires et radiologiques. L'IRSN concourt aux politiques publiques en matière de sûreté nucléaire et de protection de la santé et de l'environnement au regard des rayonnements ionisants. Organisme de recherche et d'expertise, il agit en concertation avec tous les acteurs concernés par ces politiques, tout en veillant à son indépendance de jugement.
Descriptif du poste
Société : Institut de Radioprotection et de Sûreté Nucléaire Catégorie : Stage Activité : Services Filiere : Production Metier : Electriciens, Electroniciens Lieu : Hauts-de-Seine (Île-de-France) Durée : 4 mois
Mission
Intercellular communication is vital for a tissue, enabling it to respond to a stress, such as irradiation, and to restore tissue homeostasis. To this end, cells can use microRNAs (miRNAs) as a communication mean. Their main role is to regulate the expression of certain genes in target cells.
The principal objective of this internship is to validate preliminary data identifying potential candidate miRNAs found in cell culture supernatants after irradiation of myogenic cells. To this aim, the candidate will improve the characterization of the expression profile of a panel of candidate miRNAs by quantitative PCR on samples of the murine myogenic C2C7 cell line and compare the profiles obtained between the different myogenic differentiation stages, i.e. myoblasts (proliferative cells), myocytes (differentiated cells) and myotubes (differentiated and polynucleated cells) exposed to different doses of irradiation.
At the end of internship, the candidate could examine the communication impact of the modulation of some of these miRNAs on the different myogenic cells, in terms of cell survival by using MTT assay and activation of DNA damage response by immunostaining.
Profil recherché
This internship will allow the selected student to improve his/her technical skills in cell biology and molecular biology. In addition, the candidate will acquire solid knowledge in exposure to ionizing radiation, as well as their consequences on eukaryotic cells.
We are seeking for highly motivated Master 2 student in biology / health sciences, with good knowledge in cellular and molecular biology.CLIQUER ICI POUR POSTULER
